Production of R-(-)-mandelic acid from mandelonitrile by Alcaligenes faecalis ATCC 8750

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Stereo-selective conversion of mandelonitrile to (R)-(−)-mandelic acid using immobilized cells of recombinant Escherichia coli

Immobilized cells of a recombinant Escherichia coli expressing nitrilase from Pseudomonas putida were used to catalyze the hydrolysis of mandelonitrile (2hydroxy-2-phenylacetonitrile) to (R)-(-)-mandelic acid. The cells had been immobilized by entrapment in an alginate matrix. Conditions for the hydrolysis reaction were optimized in shake flasks and in a packed bed reactor. In shake flasks the ...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1991

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.57.10.3028-3032.1991